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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
21/01/1998 |
Data da última atualização: |
21/01/1998 |
Autoria: |
MOREIRA, F. M. S.; HAUKKA, K.; YOUNG, J. P. W. |
Título: |
Biodiversity of rhizobia isolated from a wide range of forest legumes in Brazil. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Molecular Ecology, v. 7, p. 889-895, 1998. |
Idioma: |
Inglês |
Conteúdo: |
Tropical forests have a high diversity of plant species; are they associated with a correspondingly rich microbial flora? We addressed this question by examining the symbiotic rhizobium bacteria that nodulate a diverse pool of forest legume species in Brazil. The 44 strains studied had been isolated from 29 legume tree species representing 13 tribes including all three subfamilies of the Leguminosae, and were chosen to represent major groups from a larger sample that had previously been characterized by SDS-PAGE of total proteins. Partial 16S rRNA gene sequence was determined, corresponding to positions 44-303 in the Escherichia coli sequence. Fifteen sequences were found, including six novel ones. However, all but one of them could be assigned to a genus because they grouped closely with sequences from previously described rhizobial species. Fast-growing strains had sequences similar to Rhizobium spp., Sinorhizobium spp. or Mesorhizobium spp., while the slow-growing strains had sequences similar to Bradyrhizobium spp. One strain with an intermediate growth rate had a unique sequence which indicated that the strain might belong to the genus Azorhizobium. Although the strains showed a variety of sequences, it was surprising that these strains isolated from taxonomically very diverse host plants in previously unexplored environments were mostly very similar to strains described previously, largely from agricultural systems. |
Palavras-Chave: |
Ecologia microbiana. |
Thesagro: |
Biodiversidade; Floresta Tropical; Leguminosa; Rhizobium. |
Thesaurus Nal: |
biodiversity; legumes; microbial ecology; tropical forests. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02129naa a2200253 a 4500 001 1621042 005 1998-01-21 008 1998 bl --- 0-- u #d 100 1 $aMOREIRA, F. M. S. 245 $aBiodiversity of rhizobia isolated from a wide range of forest legumes in Brazil. 260 $c1998 520 $aTropical forests have a high diversity of plant species; are they associated with a correspondingly rich microbial flora? We addressed this question by examining the symbiotic rhizobium bacteria that nodulate a diverse pool of forest legume species in Brazil. The 44 strains studied had been isolated from 29 legume tree species representing 13 tribes including all three subfamilies of the Leguminosae, and were chosen to represent major groups from a larger sample that had previously been characterized by SDS-PAGE of total proteins. Partial 16S rRNA gene sequence was determined, corresponding to positions 44-303 in the Escherichia coli sequence. Fifteen sequences were found, including six novel ones. However, all but one of them could be assigned to a genus because they grouped closely with sequences from previously described rhizobial species. Fast-growing strains had sequences similar to Rhizobium spp., Sinorhizobium spp. or Mesorhizobium spp., while the slow-growing strains had sequences similar to Bradyrhizobium spp. One strain with an intermediate growth rate had a unique sequence which indicated that the strain might belong to the genus Azorhizobium. Although the strains showed a variety of sequences, it was surprising that these strains isolated from taxonomically very diverse host plants in previously unexplored environments were mostly very similar to strains described previously, largely from agricultural systems. 650 $abiodiversity 650 $alegumes 650 $amicrobial ecology 650 $atropical forests 650 $aBiodiversidade 650 $aFloresta Tropical 650 $aLeguminosa 650 $aRhizobium 653 $aEcologia microbiana 700 1 $aHAUKKA, K. 700 1 $aYOUNG, J. P. W. 773 $tMolecular Ecology$gv. 7, p. 889-895, 1998.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
01/09/2020 |
Data da última atualização: |
02/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SILVA, P. I. T.; SILVA JUNIOR, O. B. da; RESENDE, L. V.; SOUSA, V. A. de; AGUIAR, A. V. de; GRATTAPAGLIA, D. |
Afiliação: |
PEDRO ITALO T. SILVA, UNB; ORZENIL BONFIM DA SILVA JUNIOR, Cenargen; LUCILEIDE V. RESENDE; VALDERES APARECIDA DE SOUSA, CNPF; ANANDA VIRGINIA DE AGUIAR, CNPF; DARIO GRATTAPAGLIA, Cenargen. |
Título: |
A 3K Axiom SNP array from a transcriptomewide SNP resource sheds new light on the genetic diversity and structure of the iconic subtropical conifer tree Araucaria angustifolia (Bert.) Kuntze. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
PLoS ONE, v. 15, n. 8, e0230404, 2020. |
DOI: |
https://doi.org/10.1371/journal. pone.0230404 |
Idioma: |
Inglês |
Conteúdo: |
High-throughput SNP genotyping has become a precondition to move to higher precision and
wider genome coverage genetic analysis of natural and breeding populations of non-model
species. We developed a 44,318 annotated SNP catalog for Araucaria angustifolia, a grandiose subtropical conifer tree, one of the only two native Brazilian gymnosperms, critically endangered due to its valuable wood and seeds. Following transcriptome assembly and annotation,
SNPs were discovered from RNA-seq and pooled RAD-seq data. From the SNP catalog, an
Axiom® SNP array with 3,038 validated SNPs was developed and used to provide a comprehensive look at the genetic diversity and structure of 15 populations across the natural range of
the species. RNA-seq was a far superior source of SNPs when compared to RAD-seq in terms
of conversion rate to polymorphic markers on the array, likely due to the more efficient complexity reduction of the huge conifer genome. By matching microsatellite and SNP data on the
same set of A. angustifolia individuals, we show that SNPs reflect more precisely the actual
genome-wide patterns of genetic diversity and structure, challenging previous microsatellitebased assessments. Moreover, SNPs corroborated the known major north-south genetic
cline, but allowed a more accurate attribution to regional versus among-population differentiation, indicating the potential to select ancestry-informative markers. The availability of a public,
user-friendly 3K SNP array for A. angustifolia and a catalog of 44,318 SNPs predicted to provide ~29,000 informative SNPs across ~20,000 loci across the genome, will allow tackling still
unsettled questions on its evolutionary history, toward a more comprehensive picture of the origin, past dynamics and future trend of the species? genetic resources. Additionally, but not less
importantly, the SNP array described, unlocks the potential to adopt genomic prediction methods to accelerate the still very timid efforts of systematic tree breeding of A. angustifolia. MenosHigh-throughput SNP genotyping has become a precondition to move to higher precision and
wider genome coverage genetic analysis of natural and breeding populations of non-model
species. We developed a 44,318 annotated SNP catalog for Araucaria angustifolia, a grandiose subtropical conifer tree, one of the only two native Brazilian gymnosperms, critically endangered due to its valuable wood and seeds. Following transcriptome assembly and annotation,
SNPs were discovered from RNA-seq and pooled RAD-seq data. From the SNP catalog, an
Axiom® SNP array with 3,038 validated SNPs was developed and used to provide a comprehensive look at the genetic diversity and structure of 15 populations across the natural range of
the species. RNA-seq was a far superior source of SNPs when compared to RAD-seq in terms
of conversion rate to polymorphic markers on the array, likely due to the more efficient complexity reduction of the huge conifer genome. By matching microsatellite and SNP data on the
same set of A. angustifolia individuals, we show that SNPs reflect more precisely the actual
genome-wide patterns of genetic diversity and structure, challenging previous microsatellitebased assessments. Moreover, SNPs corroborated the known major north-south genetic
cline, but allowed a more accurate attribution to regional versus among-population differentiation, indicating the potential to select ancestry-informative markers. The availability of a public,
user-friendly 3K SNP array for A. angustif... Mostrar Tudo |
Thesagro: |
Araucária Angustifólia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215688/1/journal.pone.0230404.pdf
|
Marc: |
LEADER 02778naa a2200205 a 4500 001 2124667 005 2020-09-02 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1371/journal. pone.0230404$2DOI 100 1 $aSILVA, P. I. T. 245 $aA 3K Axiom SNP array from a transcriptomewide SNP resource sheds new light on the genetic diversity and structure of the iconic subtropical conifer tree Araucaria angustifolia (Bert.) Kuntze.$h[electronic resource] 260 $c2020 520 $aHigh-throughput SNP genotyping has become a precondition to move to higher precision and wider genome coverage genetic analysis of natural and breeding populations of non-model species. We developed a 44,318 annotated SNP catalog for Araucaria angustifolia, a grandiose subtropical conifer tree, one of the only two native Brazilian gymnosperms, critically endangered due to its valuable wood and seeds. Following transcriptome assembly and annotation, SNPs were discovered from RNA-seq and pooled RAD-seq data. From the SNP catalog, an Axiom® SNP array with 3,038 validated SNPs was developed and used to provide a comprehensive look at the genetic diversity and structure of 15 populations across the natural range of the species. RNA-seq was a far superior source of SNPs when compared to RAD-seq in terms of conversion rate to polymorphic markers on the array, likely due to the more efficient complexity reduction of the huge conifer genome. By matching microsatellite and SNP data on the same set of A. angustifolia individuals, we show that SNPs reflect more precisely the actual genome-wide patterns of genetic diversity and structure, challenging previous microsatellitebased assessments. Moreover, SNPs corroborated the known major north-south genetic cline, but allowed a more accurate attribution to regional versus among-population differentiation, indicating the potential to select ancestry-informative markers. The availability of a public, user-friendly 3K SNP array for A. angustifolia and a catalog of 44,318 SNPs predicted to provide ~29,000 informative SNPs across ~20,000 loci across the genome, will allow tackling still unsettled questions on its evolutionary history, toward a more comprehensive picture of the origin, past dynamics and future trend of the species? genetic resources. Additionally, but not less importantly, the SNP array described, unlocks the potential to adopt genomic prediction methods to accelerate the still very timid efforts of systematic tree breeding of A. angustifolia. 650 $aAraucária Angustifólia 700 1 $aSILVA JUNIOR, O. B. da 700 1 $aRESENDE, L. V. 700 1 $aSOUSA, V. A. de 700 1 $aAGUIAR, A. V. de 700 1 $aGRATTAPAGLIA, D. 773 $tPLoS ONE$gv. 15, n. 8, e0230404, 2020.
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